Biotinylated CD5 His&Avi Tag Protein, Human
SKU: 85473580453

Biotinylated CD5 His&Avi Tag Protein, Human

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Description

Biotinylated CD5 His&Avi Tag Protein, HumanProduct Specification Species Human Synonyms CD5; LEU1 Accession P06127 1 Amino Acid Sequence Arg25 Asn371, with C terminal His and Avitag Expression System HEK293 Molecular Weight 53 60kDa (Reducing) Purity 95% by SDS PAGE Endotoxin <1EU g Conjugation Biotin Tag Avi Tag, His Tag Physical Appearance Lyophilized Powder Storage Buffer PBS, pH7. 4. Reconstitution Reconstitute at 0. 1 1 mg ml according to the size in ultrapure water after rapid

Product Specification


Species Human
Synonyms CD5; LEU1
Accession P06127-1
Amino Acid Sequence Arg25-Asn371, with C-terminal His and Avitag
Expression System HEK293
Molecular Weight

53-60kDa (Reducing)
Purity ＞95% by SDS-PAGE
Endotoxin <1EU/μg
Conjugation Biotin
Tag Avi Tag, His Tag
Physical Appearance Lyophilized Powder
Storage Buffer PBS, pH7.4.
Reconstitution

Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.
Stability & Storage

· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.

· 3 months, -20 to -80℃ under sterile conditions after reconstitution.

· 1 week, 2 to 8℃ under sterile conditions after reconstitution.

· Please avoid repeated freeze-thaw cycles.
Reference

1. Bhandoola A, Bosselut R, Yu Q, Cowan ML, Feigenbaum L, Love PE, et al.. CD5-Mediated Inhibition of TCR Signaling During Intrathymic Selection and Development Does Not Require the CD5 Extracellular Domain. Eur J Immunol (2002) 32:1811.
2. Tarakhovsky A, Müller W, Rajewsky K. Lymphocyte Populations and Immune Responses in CD5-Deficient Mice. Eur J Immunol (1994) 24:1678–84.

Background

CD5, one of the earliest markers used to identify T cells, is a 67 kD transmembrane molecule that is constitutively expressed on all T cells and is a negative regulator of lymphocyte function. T-cell surface glycoprotein CD5 is also known as Lymphocyte antigen T1/Leu-1 and LEU1. CD5 is also a negative regulator of thymus cell stimulation. Lack of CD5 promotes positive selection of poorly selected thymocytes and increases negative selection of high-affinity clones. Along with its negative effect on T cell stimulation, CD5 was shown to diminish activation-induced cell death (AICD) through its interaction with casein kinase 2 (CK2).

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SKU: 85473580453

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4.6 ★★★★★
Based on 740 reviews
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Trekkintheplains
Fort Morgan, US
★★★★★ 5
Cute, functional, works great!
I LOVE this thing!! It froths the half and half for my coffee and cream for my tea so beautifully. It's fast, and easy to use and clean. Really adds to my morning routine to be able to choose a temperature and froth level to my coffee creamer. The color is so pretty and both it and the style fit right in with my kitchen decor. I've been using it for several months and haven't had any issues to far. Great item! I was afraid it would just be more clutter and gadgets I didn't need taking up counter space, but it fits right in next to my coffee maker, and I truly do use it every morning. It makes my coffee even more enjoyable, believe it or not. Love it!!
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Reviewed in the United States on April 10, 2026
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Verified Purchase
Melissa Tucker
San Leandro, US
★★★★★ 4
Solid purchase
Color: White, Color: White
I have used it often, basically daily. I love how it has a magnet stirrer and therefore is quiet. I like the stainless steel aspect. It does heat up well. It can store two regular mugs of beverage or maybe 3-4 mugs of froth. It is lightweight but doesn’t feel cheaply made- good enough quality. The froth is great- I have no complaints. I had to learn the hard way that non-homogenized milk gets grainy- weird and icky. I wish it was a little more frothy on the matcha side of things but I still use it. Also, I wish for things like hot chocolate that it got hotter. It goes to 160 on the machine but it doesn’t feel that hot when you drink it. I haven’t measured the temperature of the drink yet but it isn’t hot hot.
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Reviewed in the United States on January 2, 2026
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Verified Purchase
Metsa Lintu
Dallas, US
★★★★★ 5
It delivered on more levels than I imagined or expected
Color: White
EDIT: I've used this frother pretty much daily, sometimes more than daily, since ordering it, and it has performed very well. I make turmeric/ginger/pepper milk most nights. The way this frother performs for that use astounded me. I bought it for coffee/cocoa/mushroom drinks, then while waiting for it to arrive realized it might work with the "golden milk." First, the little frother uses a closely/precisely applied heat drawing 550w. The thick steel pot nestles down into the heating base, and the cover fits nice and snugly. So I'm not heating an entire thick saucepan perched atop an entire stove burner (1,000-1,500 w), with all the escaped heat that involves. Second, I'm not standing there with thermometer in hand, diving into the open pan of heating milk to find that perfect sweet spot around 150-155...while constantly stirring and making sure not to let it boil over. Because, third, the little frother takes care of that part, with the thermostat auto shut off. Fourth, it whisks the powders smoothly in. Manufacturer suggests starting the whisk movement in the milk, then adding the powders. They did collect a bit in the low vortex around the stem of the little assembly that holds the various whisk/stir/froth blades, but I just stuck a spoon handle in there carefully, and it redistributed easily around the magnetic-driven stirring assembly. And finally, omigosh, the lovely velvety texture! Since these spices express best with extended warming, I'm making one batch, heated and frothed to 160F, then follow that with just milk frothed to 140. While the second batch is running (there's a cool down pause in the process, hastened by rinsing the pot exterior in cold water and of course drying it before putting it on the base), the first batch is steeping the spices...so the combined result is perfect. EDIT: It did its job much faster than I expected (minutes). So I tested it as promised on my "solar generator" backup unit. My thought was that this would make my usual most efficient option for heating milk in those conditions the second most efficient: open saucepan atop a multi-watt-level portable induction cookplace on the 600w setting. And with much less cleanup at a time when water is scarce (electric well pump). We had a power outage here last week. Yep, the 550w frother ran on a Jackery 2000 for the few minutes it took to heat/froth milk--no trouble whatever and hardly put a dent in the charge. WOW. (Technically speaking it could run continuously, continuously heating, for over 3 1/2 hours on a full 2000 kWh charge.) EDIT: This isn't a reduction in stars, just an observation. The frother "tree" assembly that does the business of stirring/whisking/frothing--I thought to order a spare in the event of damage to the original. They are very hard to find online. Maestri House lists the replacement part as "out of stock." Also out of stock, replacement pot and lid. This might be a concern to some considering the tool. But it seems to be well made and I hope gives long service. EDIT: As of May 2026 the little frother is still working exceptionally well on a nearly daily basis. I purchased it in October of 2025, so that shouldn't be a surprise...but just sayin'. :^>
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Reviewed in the United States on October 27, 2025
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Verified Purchase
Guy Tingos
Boise, US
★★★★★ 5
This is the one to get
Color: Black
I find having separate controls for temperature and foam density is critical and this model nails it. The "newer model" listed in the product description eliminates the separate controls and does not function as well. This frother also has nice features such as the ability to produce cold foam and easy cleaning. It is also extremely quiet. I've tried several mid-range electric frothers and this is the best.
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Reviewed in the United States on May 11, 2026
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Verified Purchase
Mizz Road Runner
Lexington, US
★★★★★ 5
So far. so good.
Color: White
This is my 3rd frother that I purchased from Amazon. The others were brand name frothers that broken within a few months of use. I won't name here (hint: begins with an N and ends with an o) This Milk frother from Maestri however works excellent. Multiple heat and drink settings and makes the perfect foam every time. I especially like how the pitcher is removable from the electric base and can be washed with out worry. easy to use. Just read the manual to understand all the settings, makes a perfect froth in less than 2 minutes. I've only had this for a few weeks but I am impressed.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 11, 2026

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