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Ships within 48 hours · Estimated delivery Jul 11 - Jul 16
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Description
EasyStep Rat E2 (Estradiol) ELISA KitThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre coated with Rat E2 antigen, and the Rat E2 standard plate wells that pre coated using protein related techniques are provided separately. Standard Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP conjugated antibody specific to Rat E2 to each microplate well and
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat E2 antigen, and the Rat E2 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat E2 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Rat E2 in the samples is then determined by comparing the OD of the samples to the standard curve.
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