SKU: 63395618392

Porcine OBESTATIN ELISA Kit

$292.50 $325.00
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Description

Porcine OBESTATIN ELISA KitThe Porcine OBESTATIN ELISA Kit measures Obestatin in Porcine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with OB. OB in the sample or standard competes with a fixed amount of biotin labeled OB for sites on a pre coated antibody specific to OB. Free components are washed off. After adding HRP

The Porcine OBESTATIN ELISA Kit measures Obestatin in Porcine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with OB. OB in the sample or standard competes with a fixed amount of biotin-labeled OB for sites on a pre-coated antibody specific to OB. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), biotin specifically binds with streptavidin to form immune complexes. Wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of OB in the sample. The concentration of the target substance is inversely proportional to the OD450 value.

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SKU: 63395618392

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4.2 ★★★★★
Based on 2196 reviews
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Mr T
Grantham, US
★★★★★ 5
The right tool for the job.
Size: One Size, Style: Bottle Opener, Size: One Size, Style: Bottle Opener
This is the tool you want. It is exceptionally functional, well made (made in the USA, if that matters to you), and beautiful in the way that quality tools are. The thing that really sets this tool apart is that it correctly balances form vs. function: a heavy duty build (as is the point when buying a bottle opener built by a tool company) without its use as a bottle opener (as is the point when buying ANY bottle opener) being negatively impacted by said build. I also bought the Craftsman bottle cap wrench, which, in my opinion, is hugely successful as feeling like a heavy duty tool and something of a failure as a bottle opener. It's so large and heavy, it has equal mass to the bottle you're opening. Imagine your buddy who has had one too many wielding a 12 oz. steel wrench over your tile floor, countertop, toes, etc. and you'll get the idea. Lastly, the engraving and materials on this Klein tool are so attractive I had to build it a custom wall mount instead of hiding it away in a drawer or tool chest. I will be buying a second for the office.
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Reviewed in the United States on March 20, 2016
C
Verified Purchase
Christina
New York, US
★★★★★ 5
Solid Quality
Size: One Size, Style: Bottle Opener, Size: One Size, Style: Bottle Opener
Solid quality!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 31, 2026
O
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Opinion
Chelsea, US
★★★★★ 5
Crack them bottles with Klein
Size: One Size, Style: Bottle Opener
Use regular for bottled coke. Comes in handy!
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Reviewed in the United States on September 12, 2025
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Verified Purchase
Chris White
Dallas, US
★★★★★ 4
Good Tool
Size: One Size, Style: Bottle Opener
This thing is great at opening bottles, and it has a Klein handle. Perfect for the electrician in your life, even if that's yourself. I had to knock off a star because the handle is not quite as good as other Klein tools. The plastic is not as clear. The TIP-IDENT bottlecap marking is off-center and not fully printed. The bottle opener sticks out at a slight angle. This does not affect the performance, but it does detract from the novelty somewhat.
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Reviewed in the United States on March 10, 2023
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Verified Purchase
James C. Higgins
Phoenix, US
★★★★★ 5
Polyglo mit
Exact polyglo applicator mit. Works perfect!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 19, 2026

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